Porous layer open tubular columns with immobilized trypsin for protein digestion

0422787 - ÚIACH 2014 RIV CZ eng C - Conference Paper (international conference)
Knob, R. - Křenková, Jana - Petr, J. - Foret, František
Porous layer open tubular columns with immobilized trypsin for protein digestion.
Chemické Listy. Roč. 107, Issue s3.. Praha: Česká společnost chemická, 2013, s. 382-384. ISSN 0009-2770. E-ISSN 1213-7103.
[CECE Junior 2013. Brno (CZ), 12.11.2013-13.11.2013]
R&D Projects: GA MŠMT(CZ) EE2.3.20.0182
Grant - others:GA AV ČR(CZ) M200311201
Institutional support: RVO:68081715
Keywords : monolith * enzymes * digestion
Subject RIV: CB - Analytical Chemistry, Separation

We have developed a monolithic porous layer open tubular (PLOT) column with immobilized trypsin for protein digestion. The PLOT column was prepared in a 10 (mikro)m ID fused silica capillary. Trypsin was immobilized on the monolithic surface and the developed enzyme reactor was used for protein digestion followed by on-line ESI/MS analysis.
Permanent Link: http://hdl.handle.net/11104/0228928