Innovative technique for the direct determination of proteins in calcified aortic valves

0421182 - ÚTAM 2014 RIV DE eng J - Journal Article
Coufalová, L. - Kučková, Š. - Velčovská, M. - Zeman, Antonín - Šmíd, M. - Havelcová, Martina - Hynek, R.
Innovative technique for the direct determination of proteins in calcified aortic valves.
Analytical and Bioanalytical Chemistry. Roč. 405, č. 27 (2013), s. 8781-8787. ISSN 1618-2642. E-ISSN 1618-2650
Institutional support: RVO:68378297 ; RVO:67985891
Keywords : aortic stenosis * degenerative (calcification–sclerotic) changes * enzymatic cleavage * mass spectrometry
Subject RIV: CB - Analytical Chemistry, Separation; DD - Geochemistry (USMH-B)
Impact factor: 3.578, year: 2013
http://rd.springer.com/article/10.1007%2Fs00216-013-7306-2

Aortal valve mineralization very frequently causes a genesis of aortic stenosis, which is the most often surgically treated heart disease. Hydroxyapatite deposits have been identified as one of the causes leading to the loss of elasticity of the aortic valves. It is known that phosphates/calcium is accumulated in valve tissues during mineralization, but the mechanism of this process remains unclear. The work is focused mainly on the study of protein composition of mineralized aortic valves by nano-liquid chromatography electrospray ionization in a quadrupole orthogonal acceleration time-offlight mass spectrometry. New methodological approach based on direct enzymatic digestion of proteins contained in hydroxyapatite deposits was developed for the study of pathological processes connected with osteogenesis.
Permanent Link: http://hdl.handle.net/11104/0227751