Environmental scanning electron microscope for observation in live nature

0421180 - ÚPT 2014 JP eng C - Conference Paper (international conference)
Neděla, Vilém - Tihlaříková, Eva - Shiojiri, M.
Environmental scanning electron microscope for observation in live nature.
69th Annual Meeting of the Japanese Society of Microscopy. Osaka: The Japanese Society of Microscopy, 2013.
[Annual Meeting of the Japanese Society of Microscopy /69./. Osaka (JP), 20.05.2013-22.05.2013]
Institutional support: RVO:68081731
Keywords : ESEM * AQUASEM II * biological samples * in-situ experiments
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering

Observation of biological alive samples is one of the biggest dreams of electron microscopy. Biological samples are commonly chemically fixed and dried, sputter-coated or rapidly frozen, and observed under vacuum in TEM or SEM. For overcoming the problem of charging and approaching the possibility to observe samples in their native state, many trials have been performed using a gas environment in electron microscopes such as environmental scanning electron microscope (ESEM). Even though a possibility to observe live organisms in the ESEM was reported, the long-term study of living animal manifesting its life by a movement has not yet been achieved. We have developed a new ESEM which allows us to in-situ observe samples at high pressure environments (~2500 Pa) of various gases. The details of our microscope are shown in another presentation. Here, we demonstrate some observations of native or live samples such as plant samples, tissue samples and animals moving in water.
Permanent Link: http://hdl.handle.net/11104/0227602