The variability of the large genomic segment of Tahyna orthobunyavirus and an all-atom exploration of its anti-viral drug resistance

0420965 - BC 2014 RIV NL eng J - Journal Article
Kilian, Patrik - Valdés, James J. - Lecina-Casas, D. - Chrudimský, T. - Růžek, Daniel
The variability of the large genomic segment of Tahyna orthobunyavirus and an all-atom exploration of its anti-viral drug resistance.
Infection, Genetics and Evolution. Roč. 20, 2013-Dec (2013), s. 304-311. ISSN 1567-1348. E-ISSN 1567-7257
R&D Projects: GA ČR GAP502/11/2116; GA MŠMT(CZ) EE2.3.30.0032
Institutional support: RVO:60077344
Keywords : Tahyna virus * Orthobunyavirus * California complex * Genetic variability * Large genomic segment
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.264, year: 2013

Tahyna virus (TAHV), a member of the Bunyaviridae family (California complex), is an important but neglected human mosquito-borne pathogen. The virus genome is composed of three segments, i.e., small (S), medium (M), and large (L). Previous studies on genetic variability of viruses within the California complex were focused on S and M segments, but the L segment remains relatively unstudied. To assess the genetic variation and the relation to virus phenotype we analyzed the L segment sequences of biologically diverse TAHV strains isolated in the Czech Republic and Slovakia. Phylogenetic analysis covering all available sequences of the L segment of TAHV clearly revealed two distinguished lineages, tentatively named as "European" and "Asian". The L segment strains within the European lineage are highly conserved (identity 99.3%), whilst Asian strains are more genetically diverse (identity 97%). Based on sequence comparison with other bunyaviruses, several non-synonymous nucleotide substitutions unique for TAHV in the L segment were identified. We also identified specific residue substitutions in the endonuclease domain of TAHV compared with the La Crosse virus. Since the endonuclease domain of the La Crosse virus has been resolved, we employed an all energy landscape algorithm to analyze the ligand migration of a viral polymerase inhibitor. This allowed us to demonstrate, at the atomic level, that this viral polymerase inhibitor randomly explored the specific residue substitutions in the endonuclease domain of the TAHV L segment.
Permanent Link: http://hdl.handle.net/11104/0227486