CYCLONE-A Utility for De Novo Sequencing of Microbial Cyclic Peptides

0399080 - MBÚ 2014 RIV US eng J - Journal Article
Kavan, Daniel - Kuzma, Marek - Lemr, Karel - Schug, K. A. - Havlíček, Vladimír
CYCLONE-A Utility for De Novo Sequencing of Microbial Cyclic Peptides.
Journal of the American Society for Mass Spectrometry. Roč. 24, č. 8 (2013), s. 1177-1184. ISSN 1044-0305. E-ISSN 1879-1123
R&D Projects: GA MŠMT(CZ) ME10013; GA ČR(CZ) GAP206/12/1150
Institutional support: RVO:61388971
Keywords : Peptide * Cyclic * Sequencing
Subject RIV: CE - Biochemistry
Impact factor: 3.193, year: 2013

We have developed a de novo sequencing software tool (CYCLONE) and applied it for determination of cyclic peptides. The program uses a non-redundant database of 312 nonribosomal building blocks identified to date in bacteria and fungi (more than 230 additional residues in the database list were isobaric). The software was used to fully characterize the tandem mass spectrum of several cyclic peptides and provide sequence tags. The general strategy of the script was based on fragment ion pre-characterization to accomplish unambiguous b-ion series assignments. Showcase examples were a cyclic tetradepsipeptide beauverolide, a cyclic hexadepsipeptide roseotoxin A, a lasso-like hexapeptide pseudacyclin A, and a cyclic undecapeptide cyclosporin A. The extent of ion scrambling in smaller peptides was as low as 5 % of total ion current; this demonstrated the feasibility of CYCLONE de novo sequencing. The robustness of the script was also tested against database sets of various sizes and isotope-containing data.
Permanent Link: http://hdl.handle.net/11104/0226475