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Low-cost, easy-to-prepare magnetic chitosan microparticles for enzymes immobilization
- 1.0398969 - ÚVGZ 2014 RIV GB eng J - Journal Article
Pospišková, K. - Šafařík, Ivo
Low-cost, easy-to-prepare magnetic chitosan microparticles for enzymes immobilization.
Carbohydrate Polymers. Roč. 96, č. 2 (2013), s. 545-548. ISSN 0144-8617. E-ISSN 1879-1344
R&D Projects: GA MŠMT(CZ) LD13021
Institutional support: RVO:67179843
Keywords : Chitosan * Magnetite * Microwave irradiation * Enzymes immobilization
Subject RIV: CE - Biochemistry
Impact factor: 3.916, year: 2013
Extremely simple procedures for the preparation of magnetic chitosan enzyme carriers have been developed and used for the immobilization of lipase and -galactosidase as model enzymes. In the first case commercially available magnetite microparticles were entrapped in chitosan gel, while in the second case magnetic iron oxides microparticles were synthesized in chitosan matrix from ferrous sulfate using microwave irradiation. Immobilized enzymes showed long-term stability without leaching of enzyme from the support and enabled their repeated use without significant loss of activity.
Permanent Link: http://hdl.handle.net/11104/0226426
Number of the records: 1