Maternally recruited DCP1A and DCP2 contribute to messenger RNA degradation during oocyte maturation and genome activation in mouse

0398771 - ÚMG 2014 RIV US eng J - Journal Article
Ma, J. - Flemr, Matyáš - Strnad, Hynek - Svoboda, Petr - Schultz, R. M.
Maternally recruited DCP1A and DCP2 contribute to messenger RNA degradation during oocyte maturation and genome activation in mouse.
Biology of Reproduction. Roč. 88, č. 1 (2013), s. 1-12. ISSN 0006-3363. E-ISSN 1529-7268
R&D Projects: GA ČR(CZ) GBP305/12/G034; GA MŠMT ME09039
Institutional support: RVO:68378050
Keywords : gamete biology * maternal mRNA degradation * meiotic maturation * mRNA decapping * oocyte maturation
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.451, year: 2013

The oocyte-to-zygote transition entails transforming a highly differentiated oocyte into totipotent blastomeres and represents one of the earliest obstacles that must be successfully hurdled for continued development. Degradation of maternal mRNAs, which likely lies at the heart of this transition, is characterized by a transition from mRNA stability to instability during oocyte maturation. Although phosphorylation of the oocyte-specific RNA-binding protein MSY2 during maturation is implicated in making maternal mRNAs more susceptible to degradation, mechanisms underlying mRNA degradation during oocyte maturation remain poorly understood. We report that DCP1A and DCP2, proteins responsible for decapping mRNA, are encoded by maternal mRNAs recruited for translation during maturation via cytoplasmic polyadenylation elements located in their 30 untranslated regions. Both DCP1A and DCP2 are phosphorylated during maturation, with CDC2A being the kinase likely responsible for both, although MAPK may be involved in DCP1A phosphorylation. Inhibiting accumulation of DCP1A and DCP2 by RNA interference or morpholinos decreases not only degradation of mRNAs during meiotic maturation but also transcription of the zygotic genome. The results indicate that maternally recruited DCP1A and DCP2 are critical players in the transition from mRNA stability to instability during meiotic maturation and that proper maternal mRNA degradation must be successful to execute the oocyte-to-zygote transition.
Permanent Link: http://hdl.handle.net/11104/0228400