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Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins
- 1.0398521 - ÚOCHB 2014 RIV DE eng J - Journal Article
Daďová, Jitka - Orság, Petr - Pohl, Radek - Brázdová, Marie - Fojta, Miroslav - Hocek, Michal
Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins.
Angewandte Chemie - International Edition. Roč. 52, č. 40 (2013), s. 10515-10518. ISSN 1433-7851. E-ISSN 1521-3773
R&D Projects: GA ČR GA203/09/0317; GA ČR(CZ) GAP301/11/2076
Institutional support: RVO:61388963 ; RVO:68081707
Keywords : bioorthogonal chemistry * DNA * DNA polymerase * Michael additions * proteins
Subject RIV: CC - Organic Chemistry
Impact factor: 11.336, year: 2013
Novel bioorthogonal covalent cross-link of DNA-binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS-modified dCTP served as building block for polymerase synthesis of modified DNA which was readily conjugated with cysteine containing peptides and proteins through Michael addition.
Permanent Link: http://hdl.handle.net/11104/0226020
Number of the records: 1