Capillary Electrophoresis of Biomolecules: Methods and Protocols

0398250 - ÚOCHB 2014 RIV US eng M - Monography Chapter
Simo, C. - Cifuentes, A. - Kašička, Václav
Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies.
Capillary Electrophoresis of Biomolecules: Methods and Protocols. New York: Humana Press, 2013 - (Volpi, N.; Maccari, F.), s. 139-151. Methods in Molecular Biology, 984. ISBN 978-1-62703-296-4
R&D Projects: GA ČR(CZ) GA203/08/1428
Grant - others:AV ČR(CZ) 2008CZ0019
Institutional research plan: CEZ:AV0Z40550506
Keywords : peptides * peptidomics * proteomics * CE-MS
Subject RIV: CB - Analytical Chemistry, Separation

In this chapter, the potential of capillary electrophoresis-mass spectrometry (CE-MS) for peptide analysis is demonstrated by two strategies typically followed in analysis of these biomolecules by CE-MS. The first one is a target-based approach and it is used to detect a toxic dipeptide gama-glutamyl-S-ethenyl-cysteine in a complex matrix of legume plant. The second one is a shotgun-like methodology used for proteomic analysis; particularly, for investigation of the differences between a genetically modified variety of soybean and its conventional isogenic counterpart. These generic methods have broad applications for the analysis of peptides in a large variety of matrices, including applications in the area of proteomics and peptidomics.
Permanent Link: http://hdl.handle.net/11104/0225754