A cell-free nanofiber composite scaffold regenerated osteochondral defects in miniature pigs

0397648 - ÚEM 2014 RIV GB eng J - Journal Article
Filová, Eva - Rampichová, Michala - Litvinec, Andrej - Držík, M. - Míčková, Andrea - Buzgo, Matej - Košťáková, E. - Martinová, L. - Usvald, Dušan - Prosecká, Eva - Uhlík, J. - Motlík, Jan - Vajner, L. - Amler, Evžen
A cell-free nanofiber composite scaffold regenerated osteochondral defects in miniature pigs.
International Journal of Pharmaceutics. Roč. 447, 1-2 (2013), s. 139-149. ISSN 0378-5173. E-ISSN 1873-3476
R&D Projects: GA ČR(CZ) GAP304/10/1307
Grant - others:GA MZd(CZ) NT12156; GA MŠk(CZ) ME10145; GA UK(CZ) 96610; GA UK(CZ) 330611; GA UK(CZ) 384311; GA TA ČR(CZ) TA01011466
Program: TA
Institutional research plan: CEZ:AV0Z50390703; CEZ:AV0Z50390512
Institutional support: RVO:67985904 ; RVO:68378041
Keywords : cartilage * fibrin * nanofibers
Subject RIV: FP - Other Medical Disciplines; FP - Other Medical Disciplines (UZFG-Y)
Impact factor: 3.785, year: 2013

A novel drug delivery system was developed on the basis of the intake effect of liposomes encapsulated in PVA nanofibers. Time-controlled release of insulin and bFGF improved MSC viability in vitro. In addition, cell-free composite scaffolds containing PVA nanofibers enriched with liposomes, bFGF, and insulin were implanted into seven osteochondral defects of miniature pigs; control defects were left untreated. The cell-free composite scaffold enhanced migration of the cells into the defect, and their differentiation into chondrocytes; the scaffold was able to enhance the regeneration of osteochondral defects in minipigs.
Permanent Link: http://hdl.handle.net/11104/0225261