The structure and material composition of ossified aortic valves identified using a set of scientific methods

0397509 - ÚTAM 2014 RIV GB eng J - Journal Article
Zeman, Antonín - Šmíd, M. - Havelcová, Martina - Coufalová, L. - Kučková, S. - Velčovská, M. - Hynek, R.
The structure and material composition of ossified aortic valves identified using a set of scientific methods.
Journal of Asian Earth Sciences. Roč. 77, November (2013), s. 311-317. ISSN 1367-9120. E-ISSN 1878-5786
Grant - others:GA ČR(CZ) GA205/09/1162
Program: GA
Institutional support: RVO:68378297 ; RVO:67985891
Keywords : aortic stenosis * degenerative (calcification–sclerotic) changes * hydroxyapatite * proteins * cholesterol
Subject RIV: DB - Geology ; Mineralogy
Impact factor: 2.831, year: 2013
http://www.sciencedirect.com/science/journal/13679120/77

Degenerative aortic stenosis has become a common and dangerous disease in recent decades. This disease leads to the mineralization of aortic valves, their gradual thickening and loss of functionality. We studied the detailed assessment of the proportion and composition of inorganic and organic components in the ossified aortic valve, using a set of analytical methods applied in science: polarized light microscopy, scanning electron microscopy, X-ray fluorescence, X-ray diffraction, gas chromatography/mass spectrometry and liquid chromatography–tandem mass spectrometry. The sample valves showed the occurrence of phosphorus and calcium in the form of phosphate and calcium carbonate, hydroxyapatite, fluorapatite and hydroxy-fluorapatite, with varying content of inorganic components from 65 to 90 wt%, and with phased development of degenerative disability. The outer layers of the plaque contained an organic component with peptide bonds, fatty acids, proteins and cholesterol.
Permanent Link: http://hdl.handle.net/11104/0226945