Crystallization and preliminary X-ray crystallographic analysis of recombinant -mannosidase from Aspergillus niger

0397467 - MBÚ 2014 RIV GB eng J - Journal Article
Demo, G. - Fliedrová, Barbora - Weignerová, Lenka - Wimmerová, M.
Crystallization and preliminary X-ray crystallographic analysis of recombinant -mannosidase from Aspergillus niger.
Acta Crystallographica Section F-Structural Biology and Crystallization Communications. Roč. 69, č. 3 (2013), s. 288-291. ISSN 1744-3091. E-ISSN 2053-230X
R&D Projects: GA ČR GAP207/10/0321; GA MŠMT(CZ) 7E11011
Institutional support: RVO:61388971
Keywords : b-mannosidase * Aspergillus niger
Subject RIV: CE - Biochemistry
Impact factor: 0.568, year: 2013

B-Mannosidase (EC 3.2.1.25) is an important exoglycosidase specific for the hydrolysis of terminal B-linked mannoside in various oligomeric saccharide structures.B-Mannosidase from Aspergillus niger was expressed in Pichia pastoris and purified to clear homogeneity. B-Mannosidase was crystallized in the presence of D-mannose and the crystal diffracted to 2.41 angstrom resolution. The crystal belonged to space group P1, with unit-cell parameters a = 62.37, b = 69.73, c = 69.90 angstrom, = 108.20, = 101.51, = 103.20 degrees. The parameters derived from the data collection indicate the presence of one molecule in the asymmetric unit
Permanent Link: http://hdl.handle.net/11104/0225135