Morphology and ultrastructure of beluga (Huso huso) spermatozoa and a comparison with related sturgeons

0396994 - BC 2014 RIV NL eng J - Journal Article
Linhartová, Z. - Rodina, M. - Nebesářová, Jana - Cosson, J. - Pšenička, M.
Morphology and ultrastructure of beluga (Huso huso) spermatozoa and a comparison with related sturgeons.
Animal Reproduction Science. Roč. 137, 3-4 (2013), s. 220-229. ISSN 0378-4320. E-ISSN 1873-2232
Grant - others:GA ČR(CZ) GPP502/10/P426; GAJU(CZ) 046/2010/Z; GA MŠk(CZ) ED2.1.00/01.0024
Program: GP
Institutional support: RVO:60077344
Keywords : beluga * strugeon * spermatozoon * electron microscopy * acrosome * flagellum
Subject RIV: EA - Cell Biology
Impact factor: 1.581, year: 2013

The ultrastructure and morphology of beluga spermatozoa (Huso huso) (Acipenseridae, Chondrostei) were studied by scanning and transmission electron microscopy. Spermatozoa of 51.27 ± 4.71 μm total length (mean ± SD) were typical of sturgeon and paddlefish spermatozoa. Each consisted of an elongated nucleus (length: 5.84 ± 0.46 μm) with distinct acrosome, a cylindrical midpiece (length: 2.10 ± 0.42 μm), and a single flagellum (length: 42.21 ± 3.82 μm). The acrosome was umbrella shaped (length: 1.12 ± 0.14 μm, width: 0.87 ± 0.10 μm) with seven to nine posterolateral projections (length: 0.49 ± 0.09 μm). Three endonuclear canals, spirally arranged, traversed the nucleus length from the acrosome to the implantation fossa. The flagellum comprised an axoneme with the typical 9+2 organization of microtubules. Two flat fins were present along the flagellum parallel to the plane of the central doublet of microtubules. These fins arose at different positions, 0.57 ± 0.30 and 4.06 ± 1.32 μm from the midpiece and terminated 4.18 ± 1.09 and 4.92 ± 1.34 μm from the distal end of the flagellum. Principal component analysis revealed spermatozoan ultrastructure and morphology were similar among sturgeon species, and, although assigned to genus Huso, beluga may be closely related to the genus Acipenser.
Permanent Link: http://hdl.handle.net/11104/0230861