High Molecular Weight Forms of Mammalian Respiratory Chain Complex II

0396457 - FGÚ 2014 RIV US eng J - Journal Article
Kovářová, Nikola - Mráček, Tomáš - Nůsková, Hana - Holzerová, Eliška - Vrbacký, Marek - Pecina, Petr - Hejzlarová, Kateřina - Klučková, Katarína - Rohlena, Jakub - Neužil, Jiří - Houštěk, Josef
High Molecular Weight Forms of Mammalian Respiratory Chain Complex II.
PLoS ONE. Roč. 8, č. 8 (2013), e71869. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR(CZ) GPP303/10/P227; GA MŠMT(CZ) LL1204; GA MZd(CZ) NT12370; GA ČR(CZ) GAP301/10/1937
Institutional research plan: CEZ:AV0Z50520701
Institutional support: RVO:67985823
Keywords : supercomplexes * high molecular weihgt forms of complex II * native electrophoretic systems
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.534, year: 2013

In this study, we focused on higher molecular weight forms of CII in mammalian cells and tissues, which can be detectable after solubilisation with mild detergent digitonin and clear native electrophoresis, and on to specific interactions of CII with complexes of oxidative phosphorylation pathway or TCA cycle. Native electrophoresis in combination with immunodetection revealed existence of CIIhmw structures, which are enzymatically active and differ in electrophoretic mobility between tissues and cultured cells. These CIIhmw are unstable and readily dissociate on to CII monomer and individual subunits. Isolated defects of OXPHOS complexes have no effect on CIIhmw formation, but they are destabilised in mtDNA-depleted, rho0 cells. Immunoprecipitation and electrophoretic experiments indicate that one of the binding partners of CII could be CV, interactions with other OXPHOS or TCA cycle complexes were not detected
Permanent Link: http://hdl.handle.net/11104/0224260