The pleiotropic effect of WD-40 domain containing proteins on cellular differentiation and production of secondary metabolites in Streptomyces coelicolor

0395888 - MBÚ 2014 RIV GB eng J - Journal Article
Ulrych, Aleš - Goldová, Jana - Petříček, Miroslav - Benada, Oldřich - Kofroňová, Olga - Rampírová, Petra - Petříčková, Kateřina - Branny, Pavel
The pleiotropic effect of WD-40 domain containing proteins on cellular differentiation and production of secondary metabolites in Streptomyces coelicolor.
Molecular BioSystems. Roč. 9, č. 6 (2013), s. 1453-1469. ISSN 1742-206X
R&D Projects: GA MŠMT LH12191; GA MŠMT LH12055; GA MŠMT 2B08064
Institutional support: RVO:61388971
Keywords : AERIAL MYCELIUM FORMATION * WD-REPEAT PROTEINS * BIOSYNTHESIS GENE-CLUSTER
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.183, year: 2013

he genome of Streptomyces coelicolor encodes six potential WD-40 genes. Two of them, the wdpB (SCO5953) and the wdpC (SCO4422) genes, were studied to determine their function. Deletion of the wdpB gene resulted in a considerable decrease of aerial hyphae formation, leading to a conditionally bald phenotype, and reduced undecylprodigiosin production. In addition, the aerial hyphae of the Delta wdpB mutant strain were unusually branched and showed the signs of irregular septation and precocious lysis. Disruption of wdpC resulted in the reduction of undecylprodigiosin and delayed actinorhodin production. The Delta wdpC mutant strain showed precocious lysis of hyphae and delayed sporulation without typical curling of aerial hyphae in the early sporulation stage
Permanent Link: http://hdl.handle.net/11104/0223798