Determination of antibiotics in influents and effluents of wastewater-treatment-plants in the Czech Republic – development and application of the SPE and a UHPLC-ToFMS method

0395780 - MBÚ 2014 RIV GB eng J - Journal Article
Tylová, Tereza - Flieger, Miroslav - Olšovská, Jana
Determination of antibiotics in influents and effluents of wastewater-treatment-plants in the Czech Republic – development and application of the SPE and a UHPLC-ToFMS method.
Analytical Methods: advancing methods and applications. Roč. 5, č. 8 (2013), s. 2110-2118. ISSN 1759-9660. E-ISSN 1759-9679
R&D Projects: GA MŠMT 1M06011
Institutional support: RVO:61388971
Keywords : TANDEM MASS-SPECTROMETRY * PERFORMANCE LIQUID-CHROMATOGRAPHY * ANTIMICROBIAL CONSUMPTION ESAC
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 1.938, year: 2013

A method consisting of solid phase extraction followed by ultra-high performance liquid chromatography combined with time-of-flight mass spectrometry (UHPLC-ToFMS) has been developed for the determination of multiple-class antibiotics in influents and effluents of wastewater-treatment-plants (WWTPs). The nineteen analyzed antibiotics belong to the 5 most prescribed antibiotic classes in the Czech Republic, namely tetracyclines, macrolides, sulfonamides, fluoroquinolones, and lincosamides. The matrix-matched calibration technique with internal standard addition for each antibiotic group was employed for quantification. Two extractions employing an Oasis HLB cartridge and two injections were needed for each sample due to the different physico-chemical properties of the tested antibiotics. Prior to the extraction on the Oasis HLB SPE cartridge, the samples were adjusted to pH 4.5 and 7.5. The usefulness and versatility of the method was documented by achieving method limits of quantification up to 10 ng L-1 and recoveries >80% for most analytes
Permanent Link: http://hdl.handle.net/11104/0223694