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Preparative Purification and Isolation of Pyranoanthocyanins from Red Wine and Evaluation of Their Antioxidant Activity
- 1.0395465 - MBÚ 2014 RIV DE eng J - Journal Article
Marhol, Petr - Bednář, P. - Tománková, E. - Papoušková, B. - Stávek, J. - Híc, P. - Barták, P. - Křen, Vladimír - Balík, J.
Preparative Purification and Isolation of Pyranoanthocyanins from Red Wine and Evaluation of Their Antioxidant Activity.
Chromatographia. Roč. 76, 17-18 (2013), s. 1107-1115. ISSN 0009-5893. E-ISSN 1612-1112
R&D Projects: GA ČR(CZ) GAP301/11/0662
Institutional support: RVO:61388971
Keywords : Flash chromatography * Silica gel * Pyranoanthocyanins
Subject RIV: CC - Organic Chemistry
Impact factor: 1.370, year: 2013
Untreated silica gel was used as the stationary phase for a simple and cost-effective prepurification of 5-carboxypyranoanthocyanins from red wine enriched by the addition of pyruvic acid. The composition of the fractions obtained was analyzed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC/MS2) and the results enabled the changes in 5-carboxypyranoanthocyanin profile during preparative separation to be described and explained. Repeated purification on silica gel followed by reversed-phase separation enabled the isolation of 5-carboxypyranomalvidin-3-glucoside in high purity and represents a cheap and robust alternative to the commonly used procedures. Reversed-phase preparative chromatography with an isocratic elution [15 % acetonitrile in water (v/v)] provided sufficient resolution, showing that the addition of an acid is not necessary in anthocyanin separation. The antioxidant capacity of 5-carboxypyranomalvidin-3-glucoside was compared with malvidin-3-glucoside and a group of common polyphenols (e.g., gallic acid, quercetin, rutin, and catechin)
Permanent Link: http://hdl.handle.net/11104/0223487
Number of the records: 1