Blood Capillary Length Estimation from Three-Dimensional Microscopic Data by Image Analysis and Stereology

0395457 - FGÚ 2014 RIV US eng J - Journal Article
Kubínová, Lucie - Mao, X. W. - Janáček, Jiří
Blood Capillary Length Estimation from Three-Dimensional Microscopic Data by Image Analysis and Stereology.
Microscopy and Microanalysis. Roč. 19, č. 4 (2013), s. 898-906. ISSN 1431-9276. E-ISSN 1435-8115
R&D Projects: GA MŠMT(CZ) ME09010; GA MŠMT(CZ) LH13028; GA ČR(CZ) GAP108/11/0794
Institutional research plan: CEZ:AV0Z5011922
Institutional support: RVO:67985823
Keywords : capillaries * confocal microscopy * image analysis * length * rat brain * stereology
Subject RIV: EA - Cell Biology
Impact factor: 2.161, year: 2013

Two different rat brain regions were optically sliced by confocal microscopy and resulting 3D images processed by three types of capillary length estimation methods: (1) Stereological methods based on a computer generation of isotropic uniform random virtual test probes in 3D, either in the form of spatial grids of virtual “slicer” planes, or spherical probes. (2) Automatic method employing a digital version of the Crofton relations using the Euler characteristic of planar sections of the binary image. (3) Interactive “tracer” method for length measurement based on a manual delineation in 3D of the axes of capillary segments. The presented methods were compared in terms of their practical applicability, efficiency and precision
Permanent Link: http://hdl.handle.net/11104/0223478