A Comparative Study of Nitrilases Identified by Genome Mining

0395407 - MBÚ 2014 RIV US eng J - Journal Article
Kaplan, Ondřej - Veselá, Alicja Barbara - Petříčková, Alena - Pasquarelli, F. - Pičmanová, Martina - Rinágelová, Anna - Bhalla, T. C. - Pátek, Miroslav - Martínková, Ludmila
A Comparative Study of Nitrilases Identified by Genome Mining.
Molecular Biotechnology. Roč. 54, č. 3 (2013), s. 996-1003. ISSN 1073-6085. E-ISSN 1559-0305
R&D Projects: GA ČR(CZ) GAP504/11/0394; GA TA ČR TA01021368
Institutional support: RVO:61388971
Keywords : Nitrilase * Arylacetonitrilase * Cyanide hydratase
Subject RIV: CE - Biochemistry
Impact factor: 2.275, year: 2013

Escherichia coli strains expressing different nitrilases transformed nitriles or KCN. Six nitrilases (from Aspergillus niger (2), A. oryzae, Neurospora crassa, Arthroderma benhamiae, and Nectria haematococca) were arylacetonitrilases, two enzymes (from A. niger and Penicillium chrysogenum) were cyanide hydratases and the others (from P. chrysogenum, P. marneffei, Gibberella moniliformis, Meyerozyma guilliermondi, Rhodococcus rhodochrous, and R. ruber) preferred (hetero)aromatic nitriles as substrates. Promising nitrilases for the transformation of industrially important substrates were found: the nitrilase from R. ruber for 3-cyanopyridine, 4-cyanopyridine and bromoxynil, the nitrilases from N. crassa and A. niger for (R,S)-mandelonitrile, and the cyanide hydratase from A. niger for KCN and 2-cyanopyridine
Permanent Link: http://hdl.handle.net/11104/0223435