HUMN project initiative and review of validation, quality control and prospects for further development of automated micronucleus assays using image cytometry systems

0395233 - ÚEM 2014 RIV DE eng J - Journal Article
Fenech, M. - Kirsch-Volders, M. - Rössnerová, Andrea - Šrám, Radim - Romm, H. - Bolognesi, C. - Ramakumar, A. - Soussaline, F. - Schunck, CH. - Elhajouji, A. - Anwar, W. - Bonassi, S.
HUMN project initiative and review of validation, quality control and prospects for further development of automated micronucleus assays using image cytometry systems.
International Journal of Hygiene and Environmental Health. Roč. 216, č. 5 (2013), s. 541-552. ISSN 1438-4639. E-ISSN 1618-131X
R&D Projects: GA ČR GAP503/11/0084
Grant - others:Project NewGenesis(XE) FOOD-CT-2005-016320
Institutional support: RVO:68378041
Keywords : Micronucleus * Cytokinesis-block * Automation
Subject RIV: DN - Health Impact of the Environment Quality
Impact factor: 3.276, year: 2013

Analysis of micronuclei (MN) is a commonly used method with wide applications. As a consequence there has been a strong interest in developing automated systems to score MN, a biomarker of chromosome breakage or loss, in mammalian and human cells. The review indentifies recent progress, important limitations and steps that need to be taken into account to enable the successful universal implementation of automated micronucleus assays by image cytometry.
Permanent Link: http://hdl.handle.net/11104/0224653