The pharmacological activation of adenosine A(1) and A(3) receptors does not modulate the long- or short-term repopulating ability of hematopoietic stem and multipotent progenitor cells in mice

0394829 - BFÚ 2014 RIV NL eng J - Journal Article
Hofer, Michal - Pospíšil, Milan - Hoferová, Zuzana - Komůrková, Denisa - Páral, P. - Savvulidi, F. - Šefc, L.
The pharmacological activation of adenosine A(1) and A(3) receptors does not modulate the long- or short-term repopulating ability of hematopoietic stem and multipotent progenitor cells in mice.
Purinergic Signalling. Roč. 9, č. 2 (2013), s. 207-214. ISSN 1573-9538. E-ISSN 1573-9546
R&D Projects: GA ČR(CZ) GA305/08/0158; GA ČR(CZ) GAP303/11/0128; GA MO(CZ) OVBIOFYZ20101
Grant - others:GA MŠk(CZ) LC06044
Institutional support: RVO:68081707
Keywords : ELEVATING EXTRACELLULAR ADENOSINE * COLONY-STIMULATING FACTOR * BONE-MARROW
Subject RIV: BO - Biophysics
Impact factor: 3.510, year: 2013

This study continues our earlier findings on the hematopoiesis-modulating effects of adenosine A(1) and A(3) receptor agonists that were performed on committed hematopoietic progenitor and precursor cell populations. In the earlier experiments, N (6)-cyclopentyladenosine (CPA), an adenosine A(1) receptor agonist, was found to inhibit proliferation in the above-mentioned hematopoietic cell systems, whereas N (6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA), an adenosine A(3) receptor agonist, was found to stimulate it. The topic of this study was to evaluate the possibility that the above-mentioned adenosine receptor agonists modulate the behavior of early hematopoietic progenitor cells and hematopoietic stem cells. Flow cytometric analysis of hematopoietic stem cells in mice was employed, as well as a functional test of hematopoietic stem and progenitor cells (HSPCs).
Permanent Link: http://hdl.handle.net/11104/0223011