ZrFsy1, a High-Affinity Fructose/H+ Symporter from Fructophilic Yeast Zygosaccharomyces rouxii

0394800 - FGÚ 2014 RIV US eng J - Journal Article
Leandro, Maria José - Sychrová, Hana - Prista, C. - Loureiro-Dias, M.C.
ZrFsy1, a High-Affinity Fructose/H+ Symporter from Fructophilic Yeast Zygosaccharomyces rouxii.
PLoS ONE. Roč. 8, č. 7 (2013), e68165. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA MŠMT(CZ) LC531; GA ČR(CZ) GAP503/10/0307
Institutional research plan: CEZ:AV0Z50110509
Institutional support: RVO:67985823
Keywords : fructose transport * fructophily * Zygosaccharomyces rouxii
Subject RIV: EI - Biotechnology ; Bionics
Impact factor: 3.534, year: 2013

Zygosaccharomyces rouxii is a fructophilic yeast than can grow at very high sugar concentrations. We have identified an ORF encoding a putative fructose/H+ symporter in the Z. rouxii CBS 732 genome database. Heterologous expression of this ORF in a S. cerevisiae strain lacking its own hexose transporters (hxt-null) and subsequent kinetic characterization of its sugar transport showed it is a high-affinity low-capacity fructose/H+ symporter
Permanent Link: http://hdl.handle.net/11104/0222965