Asymmetric dimethylarginine regulates the lipopolysaccharide-induced nitric oxide production in macrophages by suppressing the activation of NF-kappaB and iNOS expression

0394792 - BFÚ 2014 RIV NL eng J - Journal Article
Pekarová, Michaela - Kubala, Lukáš - Martíšková, Hana - Binó, Lucia - Twarogová, M. - Klinke, A. - Rudolph, T.K. - Kuchtová, Z. - Kolářová, Hana - Ambrožová, Gabriela - Kuchta, R. - Kadlec, J. - Lojek, Antonín
Asymmetric dimethylarginine regulates the lipopolysaccharide-induced nitric oxide production in macrophages by suppressing the activation of NF-kappaB and iNOS expression.
European Journal of Pharmacology. Roč. 713, 1-3 (2013), s. 68-77. ISSN 0014-2999. E-ISSN 1879-0712
R&D Projects: GA ČR(CZ) GP13-40882P
Institutional research plan: CEZ:AV0Z50040702
Institutional support: RVO:68081707
Keywords : Macrophage * Methylarginine * Asymmetric dimethylarginine
Subject RIV: BO - Biophysics
Impact factor: 2.684, year: 2013

Two major effector systems are frequently implicated in the immune and endothelial cell alternations associated with inflammation. They include the enhanced production of reactive oxygen species and diminished bioavailability of nitric oxide (NO). Importantly, these processes can be regulated by endogenously produced methylarginines, inhibitors for NO derived from macrophages and endothelial cells. Therefore, the aim of this study was to show the potential pharmacological intervention of methylarginines (N-G-methyl-L-arginine, L-NMMA; N-G, N-G'-dimethyl-L-arginine-symmetric dimethylarginine, SDMA; and N-G, N-G-dimethyl-L-arginine-asymmetric dimethylarginine, ADMA) in activation of murine peritoneal (RAW 264.7) and alveolar (MHS) macrophages with lipopolysaccharide from Gram-negative bacteria (LPS).
Permanent Link: http://hdl.handle.net/11104/0222987