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Nucleobase protection strategy for gene cloning and expression

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    0393858 - ÚOCHB 2014 RIV DE eng J - Journal Article
    Kielkowski, Pavel - Brock, N. L. - Dickschat, J. S. - Hocek, Michal
    Nucleobase protection strategy for gene cloning and expression.
    Chembiochem. Roč. 14, č. 7 (2013), s. 801-804. ISSN 1439-4227. E-ISSN 1439-7633
    R&D Projects: GA ČR GA203/09/0317
    Institutional support: RVO:61388963
    Keywords : DNA * polymerase chain reaction * protecting groups * protein production * restriction endonucleases
    Subject RIV: CE - Biochemistry
    Impact factor: 3.060, year: 2013

    Protecting group chemistry meets molecular biology: Chemically modified dATP carrying a bulky triethylsilylethynyl group was used in a PCR-based synthesis of a gene internally protected against cleavage by restriction endonucleases. The unmodified flanking regions were cleaved for cloning into a plasmid which was replicated by E. coli, and used for protein production.
    Permanent Link: http://hdl.handle.net/11104/0222228

     
     
Number of the records: 1  

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