Flow cytometry for the development of biotechnological processes with microalgae

0393298 - BÚ 2014 RIV US eng J - Journal Article
Hyka, P. - Ličková, S. - Přibyl, Pavel - Melzoch, K. - Kovář, K.
Flow cytometry for the development of biotechnological processes with microalgae.
Biotechnology Advances. Roč. 31, č. 1 (2013), s. 2-16. ISSN 0734-9750. E-ISSN 1873-1899
Institutional support: RVO:67985939
Keywords : flow cytometry * fluorescence * microalga
Subject RIV: EF - Botanics
Impact factor: 8.905, year: 2013

Microalgae appear to be a sustainable source of various valuable substances as unique biotechnological production systems. This review summarizes use of flow cytometric (FCM) methods for analysing the physiological state of microalgae. Cells possessing the desired physiological or morphological features, which are detectable with or without fluorescent staining, are counted or isolated (sorted) using an FCM device. The options for implementation of FCM in the development of biotechnological processes detailed in this review are (i) analysing the chemical composition of biomass, (ii) monitoring cellular enzyme activity and cell viability, and (iii) sorting cells to isolate those overproducing the target compound or for the preparation of axenic cultures.
Permanent Link: http://hdl.handle.net/11104/0222851