Inhibition of chlorophyll biosynthesis at the protochlorophyllide reduction step results in the parallel depletion of Photosystem I and Photosystem II in the cyanobacterium Synechocystis PCC 6803

0393107 - MBÚ 2014 RIV DE eng J - Journal Article
Kopečná, Jana - Sobotka, Roman - Komenda, Josef
Inhibition of chlorophyll biosynthesis at the protochlorophyllide reduction step results in the parallel depletion of Photosystem I and Photosystem II in the cyanobacterium Synechocystis PCC 6803.
Planta. Roč. 237, č. 2 (2013), s. 497-508. ISSN 0032-0935. E-ISSN 1432-2048
R&D Projects: GA ČR GAP501/10/1000; GA ČR GBP501/12/G055; GA MŠMT(CZ) ED2.1.00/03.0110
Institutional support: RVO:61388971
Keywords : Chlorophyll biosynthesis * Cyanobacteria * Photosystems
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.376, year: 2013

In most oxygenic phototrophs, including cyanobacteria, two independent enzymes catalyze the reduction of protochlorophyllide to chlorophyllide, which is the penultimate step in chlorophyll (Chl) biosynthesis. One is light-dependent NADPH:protochlorophyllide oxidoreductase (LPOR) and the second type is dark-operative protochlorophyllide oxidoreductase (DPOR). To clarify the roles of both enzymes, we assessed synthesis and accumulation of Chl-binding proteins in mutants of cyanobacterium Synechocystis PCC 6803 that either completely lack LPOR or possess low levels of the active enzyme due to its ectopic regulatable expression. The LPOR-less mutant grew photoautotrophically in moderate light and contained a maximum of 20 % of the wild-type (WT) Chl level
Permanent Link: http://hdl.handle.net/11104/0221863