Duration of the first steps of the human rRNA processing

0392695 - BFÚ 2014 RIV US eng J - Journal Article
Popov, A. - Smirnov, E. - Kováčik, L. - Raška, O. - Hagen, G. - Stixová, Lenka - Raška, I.
Duration of the first steps of the human rRNA processing.
Nucleus. Roč. 4, č. 2 (2013), s. 134-141. ISSN 1949-1034. E-ISSN 1949-1042
R&D Projects: GA ČR(CZ) GBP302/12/G157; GA MŠMT(CZ) EE2.3.30.0030
Grant - others:GA ČR(CZ) GAP302/12/1885
Institutional research plan: CEZ:AV0Z50040702
Institutional support: RVO:68081707
Keywords : rRNA processing * cleavage * half-life time
Subject RIV: BO - Biophysics
Impact factor: 3.148, year: 2013

Processing of rRNA in mammalian cells includes a series of cleavages of the primary 47S transcript and results in producing three rRNAs: 18S, 28S and 5.8S. The sequence of the main processing events in human cells has been established, but little is yet known about the dynamics of this process, especially the dynamics of its early stages. In the present study, we used real-time PCR to measure levels of pre-rRNA after inhibition of transcription with actinomycin D. Thus we could estimate the half-life time of rRNA transcripts in two human-derived cell lines, HeLa and LEP (human embryonic fibroblasts), as well as in mouse NIH 3T3 cells. The primary transcripts seemed to be more stable in the human than in the murine cells. Remarkably, the graphs in all cases showed more or less pronounced lag phase, which may reflect preparatory events preceding the first cleavage of the pre-rRNA.
Permanent Link: http://hdl.handle.net/11104/0221506