Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study

0392660 - BFÚ 2014 RIV GB eng J - Journal Article
Palacký, J. - Vorlíčková, Michaela - Kejnovská, Iva - Mojzeš, P.
Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study.
Nucleic Acids Research. Roč. 41, č. 2 (2013), s. 1005-1016. ISSN 0305-1048. E-ISSN 1362-4962
R&D Projects: GA ČR(CZ) GA202/09/0193; GA ČR(CZ) GAP205/12/0466
Grant - others:GA ČR(CZ) ED1.1.00/02.0068
Program: ED
Institutional research plan: CEZ:AV0Z50040702
Institutional support: RVO:68081707
Keywords : INTRAMOLECULAR G-QUADRUPLEX * K+ SOLUTION * B-DNA
Subject RIV: BO - Biophysics
Impact factor: 8.808, year: 2013

DNA concentration has been recently suggested to be the reason why different arrangements are revealed for K+-stabilized human telomere quadruplexes by experimental methods requiring DNA concentrations differing by orders of magnitude. As Raman spectroscopy can be applied to DNA samples ranging from those accessible by absorption and CD spectroscopies up to extremely concentrated solutions, gels and even crystals; it has been used here to clarify polymorphism of a core human telomeric sequence G(3)(TTAG(3))(3) in the presence of K+ and Na+ ions throughout wide range of DNA concentrations. We demonstrate that the K+-structure of G(3)(TTAG(3))(3) at low DNA concentration is close to the antiparallel fold of Na+-stabilized quadruplex. On the increase of G(3)(TTAG(3))(3) concentration, a gradual transition from antiparallel to intramolecular parallel arrangement was observed, but only for thermodynamically equilibrated K+-stabilized samples.
Permanent Link: http://hdl.handle.net/11104/0221494