New analogs of the CART peptide with anorexigenic potency: The importance of individual disulfide bridges

0391965 - ÚOCHB 2014 RIV US eng J - Journal Article
Blechová, Miroslava - Nagelová, Veronika - Žáková, Lenka - Demianova, Zuzana - Železná, Blanka - Maletínská, Lenka
New analogs of the CART peptide with anorexigenic potency: The importance of individual disulfide bridges.
Peptides. Roč. 39, January (2013), s. 138-144. ISSN 0196-9781. E-ISSN 1873-5169
R&D Projects: GA ČR GAP303/10/1368
Institutional support: RVO:61388963
Keywords : CART peptide analogs * sulfitolysis * PC12 cells * binding * food intake
Subject RIV: CE - Biochemistry
Impact factor: 2.614, year: 2013

The CART (cocaine- and amphetamine-regulated transcript) peptide is an anorexigenic neuropeptide that acts in the hypothalamus. The receptor and the mechanism of action of this peptide are still unknown. In our previous study, we showed that the CART peptide binds specifically to PC12 rat pheochromocytoma cells in both the native and differentiated into neuronal phenotype. Two biologically active forms, CART(55-102) and CART(61-102), with equal biological activity, contain three disulfide bridges. To clarify the importance of each of these disulfide bridges in maintaining the biological activity of CART(61-102), an Ala scan at particular S-S bridges forming cysteines was performed, and analogs with only one or two disulfide bridges were synthesized. In this study, a stabilized CART(61-102) analog with norleucine instead of methionine at position 67 was also prepared and was found to bind to PC12 cells with an anorexigenic potency similar to that of CART(61-102). The binding study revealed that out of all analogs tested, [Ala(68,86)]CART(61-102), which contains two disulfide bridges (positions 74-94 and 88-101), preserved a high affinity to both native PC12 cells and those that had been differentiated into neurons. In food intake and behavioral tests with mice after intracerebroventricular administration, this analog showed strong and long-lasting anorexigenic potency. Therefore, the disulfide bridge between cysteines 68 and 86 in CART(61-102) can be omitted without a loss of biological activity, but the preservation of two other disulfide bridges and the full-length peptide are essential for biological activity.
Permanent Link: http://hdl.handle.net/11104/0220973