FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion

0391710 - FGÚ 2014 RIV IE eng J - Journal Article
Ostašov, Pavel - Sýkora, Jan - Brejchová, Jana - Olžyńska, Agnieszka - Hof, Martin - Svoboda, Petr
FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion.
Chemistry and Physics of Lipids. 167-168, FEB-MAR (2013), s. 62-69. ISSN 0009-3084. E-ISSN 1873-2941
R&D Projects: GA ČR(CZ) GAP207/12/0919
Institutional research plan: CEZ:AV0Z50110509
Institutional support: RVO:67985823 ; RVO:61388955
Keywords : cholesterol depletion * beta-Cyclodextrin * 22-NBD-cholesterol * 25-NBD-cholesterol * FLIM studies * intact HEK293 cells
Subject RIV: CE - Biochemistry; CF - Physical ; Theoretical Chemistry (UFCH-W)
Impact factor: 2.593, year: 2013

The observation that 22-NBD-cholesterol appears to be indifferent to the changes in the membrane packing in living cells is in agreement with previous studies in model membranes. However, our data indicate that the alternation of plasma membrane structure induced by decrease of cholesterol level by beta-CDX makes the membrane environment of NBD moiety of 25-NBD-cholesterol probe a significantly more hydrated. This finding not only encourages using 25-NBD-cholesterol in living cells, but also demonstrates that previously drawn discouraging conclusions on the use of 25-NBD-cholesterol in model membranes are not valid for living cells
Permanent Link: http://hdl.handle.net/11104/0220714