Imaging of Protein Synthesis: In Vitro and In Vivo Evaluation of Sc-44-DOTA-Puromycin

0391532 - ÚJF 2014 RIV US eng J - Journal Article
Eigner, Sebastian - Beckford, Denis R. - Fellner, M. - Loktionova, N. - Piel, M. - Lebeda, Ondřej - Rosch, F. - Ross, T. L. - Eigner-Henke, Kateřina
Imaging of Protein Synthesis: In Vitro and In Vivo Evaluation of Sc-44-DOTA-Puromycin.
Molecular Imaging and Biology. Roč. 15, č. 1 (2013), s. 79-86. ISSN 1536-1632. E-ISSN 1860-2002
R&D Projects: GA MŠMT 2B06165
Institutional support: RVO:61389005
Keywords : protein synthesis * Scandium-44 * DOTA-Pur * therapy control * mu PET * preclinical imaging
Subject RIV: FR - Pharmacology ; Medidal Chemistry
Impact factor: 2.869, year: 2013
http://link.springer.com/content/pdf/10.1007%2Fs11307-012-0561-3

he purpose of this study was to investigate whether Sc-44-labeled puromycin can be utilized for imaging of protein synthesis in vivo. For micro-positron emission tomographic (mu PET) studies, 20-25 MBq of [Sc-44]-DOTA-puromycin was administered to tumor-bearing rats, and animals were scanned for 1 h dynamically. Results were further validated by dissecting organs and tissues of the animals after the measurement and in vitro blocking experiments using puromycin or cycloheximide to block protein synthesis. mu PET images of tumor-bearing rats showed significant tumor uptake of [Sc-44]-DOTA-puromycin and a clear-cut tumor visualization. In both blocking experiments, cellular uptake of [Sc-44]-DOTA-puromycin ([Sc-44]-DOTA-Pur) could be suppressed by blocking protein synthesis. We report for the first time successful mu PET imaging with Sc-44 obtained from a Ti-44/Sc-44 generator, as well as noninvasive mu PET imaging of ribosomal activity, respectively protein synthesis, with a puromycin-based radiopharmaceutical and the direct correlation between cellular uptake of [Sc-44]-DOTA-Pur and protein synthesis.
Permanent Link: http://hdl.handle.net/11104/0220552