Microfluidic devices for analysis and active optical sorting of individual cells

0391348 - ÚPT 2014 RIV CZ eng J - Journal Article
Ježek, Jan - Pilát, Zdeněk - Šerý, Mojmír - Kaňka, Jan - Samek, Ota - Bernatová, Silvie - Zemánek, Pavel
Microfluidic devices for analysis and active optical sorting of individual cells.
Jemná mechanika a optika. Roč. 58, č. 2 (2013), s. 55-59. ISSN 0447-6441
R&D Projects: GA MPO FR-TI1/433; GA MŠMT ED0017/01/01; GA ČR GAP205/11/1687
Institutional support: RVO:68081731
Keywords : microfluidic * cell sorting * optical tweezers * Raman spectroscopy
Subject RIV: EI - Biotechnology ; Bionics

We have constructed a device for active optical manipulation and Raman spectral analysis in a microfluidic environment. This system alllows effecient, nondestructive and contactless sorting of biological samples based on the Raman spectroscopie characteristics of individual living cells. The sorting platform used a combination of fast galvano-optics laser steering system and specially designed microfluidic chips. We used X shaped channels with two input and output ports as well as several other variants with different flow characteristics. The steerable trapping laser beam was designed to move the cell to the specified locations and confine the cell for the time period needed for the analysis.
Permanent Link: http://hdl.handle.net/11104/0225231