Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop

0391203 - BC 2013 RIV US eng J - Journal Article
Vlasák, Josef - Bříza, Jindřich - Pavingerová, Daniela - Modlinger, R. - Knížek, M. - Malá, J.
Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop.
African Journal of Biotechnology. Roč. 11, č. 86 (2012), s. 15236-15240. ISSN 1684-5315
R&D Projects: GA MZe QH71290; GA ČR(CZ) GAP502/11/1471
Institutional research plan: CEZ:AV0Z50510513
Institutional support: RVO:60077344
Keywords : Bacillus thuringiensis * Ips typographus * Picea abies
Subject RIV: EI - Biotechnology ; Bionics
Impact factor: 0.573, year: 2010

Bacillus thuringiensis Cry3A gene was redesigned for high expression in Norwegian spruce and the sequence was slightly modified to allow for simple N- and C- terminal deletions and domain II loop 1 exchange for synthetic oligos. Modified Cry3A toxins from 13 variants of the synthetic gene were expressed in Escherichia coli BL21 and their toxicity on spruce bark beetle larvae was tested using spruce bark sandwiches. Mutant toxins with N-terminal deletion and loop 1 duplication showed increased toxicity.
Permanent Link: http://hdl.handle.net/11104/0220127