Diversity and organization of genes for aerobic degradation of aromatic compounds under high environmental pollution

0390904 - MBÚ 2013 CZ eng A - Abstract
Josefiová, Jiřina - Brennerová, Mária
Diversity and organization of genes for aerobic degradation of aromatic compounds under high environmental pollution.
International Symposium on Biosorption and Bioremediation /5./. Praha: VSCHT, 2012. s. 162-162. ISBN 978-80-7080-819-1.
[International Symposium on Biosorption and Bioremediation /5./. 24.06.2012-28.06.2012, Praha]
R&D Projects: GA MŠMT 1M06011
Institutional research plan: CEZ:AV0Z50200510
Keywords : PCR screening
Subject RIV: EE - Microbiology, Virology

Metagenomic libraries were constructed from soil DNA representing a heavily polluted with petroleum hydrocarbons area. PCR screening approach allowed to identify 50% of all metagenomic clones as carrying EXDO-encoding genes of three major known phylogenetic groups described in cultured bacterial degraders. Unclassified clones were analyzed by transposon mutagenesis and pyrosequencing, and uncommon for the classical catabolic pathways organization - from solitary localization to multiple arrays of xenologous genes, was found.
Permanent Link: http://hdl.handle.net/11104/0219787