New Environmental Scanning Electron Microscopy and Observation of Live Nature

0390639 - ÚPT 2013 RIV TH eng C - Conference Paper (international conference)
Neděla, Vilém - Tihlaříková, Eva - Makoto, S.
New Environmental Scanning Electron Microscopy and Observation of Live Nature.
MST30 2013. Proceedings of the 30th Annual Conference of the Microscopy Society of Thailand. Chanthaburi: Microscopy Society of Thailand, 2013, s. 19-20. ISBN 978-974-7533-16-3.
[MST30 2013. The 30th Annual Conference of the Microscopy Society of Thailand. Chanthaburi (TH), 23.01.2013-25.01.2013]
R&D Projects: GA ČR GAP102/10/1410; GA MŠMT EE.2.3.20.0103
Institutional support: RVO:68081731
Keywords : Environmental Scanning Electron Microscopy * Observation of Live Nature
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering

Observation of biological alive samples is one of the biggest dreams of electron microscopy. However, biological samples are commonly chcmically fixed and dried, sputter-coated on their surfaces with thin conductive film or rapidly frozen, and observed by electron irradiation under vacuum in the conventional transmission electron microscopes (TEMs) or scanning electron microscopes (SEMs). For overcoming the problem of charging and approaching the possibility to observe samples in their nature state, scientists have tried to use a gas environment in electron microscopes such as environmental scanning electron microscopc (ESEM), and many results of samples observation in various environmental atmospheres were reported. Unfortunately, they have not succeeded in the observing biological specimens alive.
Permanent Link: http://hdl.handle.net/11104/0219660