Biodegradation of PCBs by ligninolytic fungi and characterization of the degradation products

0390086 - MBÚ 2013 RIV GB eng J - Journal Article
Čvančarová, Monika - Křesinová, Zdena - Filipová, Alena - Covino, S. - Cajthaml, Tomáš
Biodegradation of PCBs by ligninolytic fungi and characterization of the degradation products.
Chemosphere. Roč. 88, č. 11 (2012), s. 1317-1323. ISSN 0045-6535. E-ISSN 1879-1298
R&D Projects: GA ČR GA525/09/1058
Institutional support: RVO:61388971
Keywords : Polychlorinated biphenyls * PCBs * Biodegradation
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.137, year: 2012

The aim of the present study was to compare the degrading capabilities of eight ligninolytic fungal representatives towards a technical mixture of polychlorinated biphenyls (Delor 103). Axenic cultures of the fungi, either in complex or N-limited liquid media, were spiked with the technical mixture of Delor 103. All of the fungal strains were able to degrade the pollutant significantly after 6 weeks of incubation in both media. Outstanding results were achieved by the treatment with Pleurotus ostreatus, which removed 98.4% and 99.6% of the PCB mixture in complex and mineral media, respectively. This fungus was the only one capable of breaking down penta- and hexachlorinated biphenyls in the complex medium. Ecotoxicological assays performed with the luminescent bacterium Vibrio fischeri demonstrated that all of the fungal strains employed in this study were able to remove the toxicity only temporarily (e.g., after 28 d of incubation), while P. ostreatus was capable of suppressing the toxicity associated to PCBs along the whole incubation period in both media. We also performed an extensive set of qualitative GC/MS analyses and chlorinated derivatives of hydroxy- and methoxy-biphenyls were detected along with monoaromatic structures, i.e. chlorobenzoic acids,chlorobenzaldehydes and chlorobenzyl alcohols
Permanent Link: http://hdl.handle.net/11104/0219037