A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous

0389788 - BFÚ 2013 RIV US eng J - Journal Article
Kovařík, Aleš - Lim, K. Y. - Součková Skalická, Kamila - Matyášek, Roman - Leitch, A. R.
A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous.
Botanical Journal of the Linnean Society. Roč. 170, č. 3 (2012), s. 459-471. ISSN 0024-4074. E-ISSN 1095-8339
R&D Projects: GA ČR(CZ) GAP501/10/0208
Institutional support: RVO:68081707
Keywords : cell biology * cell cycle * evolution
Subject RIV: BO - Biophysics
Impact factor: 2.589, year: 2012

Nicotiana tabacum (tobacco, 2n?=?4x?=?48) is an allotetraploid with 24 S-genome chromosomes (from a diploid related to N.?sylvestris) and 24 T-genome chromosomes (from a diploid related to N.?tomentosiformis). The BY-2 suspension cell culture, derived from N.?tabacum cultivar Bright Yellow 2, has been used extensively for research in molecular and cell biology for almost 40 years; a Web of Knowledge search reveals that it has been used over 150 times since 2008 alone, largely for cell cycle and plant physiology studies. However, we show that this culture is unstable and, as with other long-term cultures, exists as a community of cells with different karyotypes reflected in different chromosome numbers, morphologies and distributions of satellite repeats.
Permanent Link: http://hdl.handle.net/11104/0218648