Photoacclimation of Phaeodactylum tricornutum (Bacillariophyceae) cultures grown outdoors in photobioreactors and open ponds

0389649 - MBÚ 2013 RIV GB eng J - Journal Article
Torzillo, G. - Faraloni, C. - Silva, A. M. - Kopecký, Jiří - Pilný, Jan - Masojídek, Jiří
Photoacclimation of Phaeodactylum tricornutum (Bacillariophyceae) cultures grown outdoors in photobioreactors and open ponds.
European Journal of Phycology. Roč. 47, č. 2 (2012), s. 169-181. ISSN 0967-0262. E-ISSN 1469-4433
R&D Projects: GA ČR GA521/09/0656; GA MŠMT(CZ) ED2.1.00/03.0110
Institutional support: RVO:61388971
Keywords : chlorophyll fluorescence * diatom * culture
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.912, year: 2012

The acclimation to high light of Phaeodactylum tricornutum cultures grown outdoors both in an open pond and in tubular photobioreactors (PBRs) was studied by means of chlorophyll fluorescence, pigment analysis and growth. Cultures grown in PBRs (5-cm tube diameter) at two biomass concentrations (0.3 and 0.6 g l(-1)) were compared with a culture grown in a 10-cm deep open pond (0.3 g l(-1)). Therefore, the performance of the cultures was compared on the basis of both the same biomass concentration and areal density. Cultures grown at 0.3 g l(-1) in PBRs experienced the highest light stress, which resulted in dramatic changes in both chlorophyll fluorescence and photosynthesis parameters, and in low areal productivity. In this culture, the F-v/F-m ratio was 0.5 in the morning and dropped to about 0.1 within 1 h of exposure to bright sunlight. Similar behaviour was observed with the measurements of rETR, and the initial slope (alpha(ETR)) of the photosynthesis curve, while the saturation irradiance parameter (I-k) increased about four-fold compared to the morning value
Permanent Link: http://hdl.handle.net/11104/0218505