Role of vinculin in meiosis during the mouse spermatobenesis

0389002 - ÚMG 2014 JP eng C - Conference Paper (international conference)
Rohožková, Jana - Hozák, Pavel
Role of vinculin in meiosis during the mouse spermatobenesis.
14th International Congress of Histochemistry and Cytochemistry. Kyoto: International Federation of Societies for Histochemistry and Cytochemistry, 2012.
[14th International Congress of Histochemistry and Cytochemistry. Kyoto (JP), 26.08.2012-29.08.2012]
Grant - others:FEI(US) 250694
Institutional support: RVO:68378050
Keywords : Vinculin * meiosis * synaptonemal complex
Subject RIV: EB - Genetics ; Molecular Biology

Vinculin (VCL) is a member of proteins described as molecules responsible to sense the mechanical properties of the extracellular environment, and to act as a regulator of mechanical stress in addition to its function as a mechano-coupling protein. Vinculin is the main component of the focal adhesions establishing cell-cell and cell-matrix interaction. Disrupting of vinculin leads to deregulation of mentioned interactions and increased cell migration in a 3D environment. This, in turn, is considered prerequisite for the development of malignant tumors. Till present the presence of VCL was described in U2OS at ultrastructural level. Except prove of its presence in interphase cell we observed localization of vinculin during the meiosis. Immunofluorescence double labeling of frozen sections of the mouse testis with rabbit polyclonal anti-SMC3 (main component of synaptonemal complex created between homologous paired chromosome) and goat-polyclonal anti-VCL antibody. Co-loclaization of both proteins was done also on the spermatocytes spreads. Using the immunofluorescence method we have described presence of vinculin on the mouse testes cryo-sections (on the basement of Ductus seminiferi) and on the single meiotic cell spreads. VCL presence dynamic localization within the creation of tetrad homolog in zygotene (in cloe vicinity to the centromers), during pachytene directly co-localizing with the centromere and decorating the synaptonemal commplex aboard. Localization of VCL in the centromer is conserved till the end of meiosis I producing secondary spermatocytes. During the meiotic division cell undergoes extensive changes in shape, size and movement. The cytoskeleton, which comprises actin, microtubules and intermediate filaments, is believed to function in these cellular events. VCL is showing to be a reasonable part of the chromosome dynamic machinery during the meiosis. However concrete role of VCL still remains unclear.
Permanent Link: http://hdl.handle.net/11104/0228421