Mechanistic Study of 17 alpha-Ethinylestradiol Biodegradation by Pleurotus ostreatus: Tracking of Extracelullar and Intracelullar Degradation Mechanisms

0388819 - MBÚ 2013 RIV US eng J - Journal Article
Křesinová, Zdena - Moeder, M. - Ezechiáš, Martin - Svobodová, Kateřina - Cajthaml, Tomáš
Mechanistic Study of 17 alpha-Ethinylestradiol Biodegradation by Pleurotus ostreatus: Tracking of Extracelullar and Intracelullar Degradation Mechanisms.
Environmental Science and Technology. Roč. 46, č. 24 (2012), s. 13377-13385. ISSN 0013-936X. E-ISSN 1520-5851
R&D Projects: GA TA ČR TA01020804; GA ČR GAP503/10/0408
Institutional support: RVO:61388971
Keywords : WHITE-ROT FUNGI * ENDOCRINE-DISRUPTING CHEMICALS * PERSONAL CARE PRODUCTS
Subject RIV: EE - Microbiology, Virology
Impact factor: 5.257, year: 2012

The white rot fungus Pleurotus ostreatus is able to completely remove the synthetic hormone 17 alpha-ethinylestradiol (EE2, 200 mu g in 20 mL) from a liquid complex or mineral medium in 3 or 14 days, respectively. Its efficiency has also been documented in the removal of estrogenic activity that correlated with the EE2 degradation. A set of in vitro experiments using various cellular and enzyme fractions has been performed and the results showed that EE2 was degraded by isolated laccase (about 90% within 24 h). The degradation was also tested with concentrated extracellular liquid where degradation reached 50% mainly due to the laccase activity; however, after a supplementation with H2O2 and Mn2+, residual manganese-dependent peroxidase activities (40 times lower than Lac) raised the degradation to 100%. Moreover, the intracellular fraction and also laccase-like activity associated with fungal mycelium were found to be efficient in the degradation too
Permanent Link: http://hdl.handle.net/11104/0217727