Expression of the skeletal calsequestrin isoform in normal and regenerated skeletal muscles and in the hearts of rats with altered thyroid status

0388552 - FGÚ 2013 RIV CZ eng J - Journal Article
Soukup, Tomáš - Sulimenko, Vadym - Marková, Vladimíra - Kopecká, Kateřina - Zachařová, Gisela - Paleček, Jiří
Expression of the skeletal calsequestrin isoform in normal and regenerated skeletal muscles and in the hearts of rats with altered thyroid status.
Physiological Research. Roč. 61, č. 6 (2012), s. 575-586. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA ČR(CZ) GA304/08/0256; GA ČR(CZ) GA305/09/1228; GA ČR(CZ) GBP304/12/G069
Grant - others:EC(XE) Myores 511978
Institutional research plan: CEZ:AV0Z50110509; CEZ:AV0Z50520701
Institutional support: RVO:67985823 ; RVO:68378050
Keywords : skeletal calsequestrin * calcium and muscle contraction * thyroid hormones * western blot and qRT-PCR analysis * muscle transplantation * fast and slow muscle fibers
Subject RIV: EA - Cell Biology
Impact factor: 1.531, year: 2012

We found that the protein and mRNA levels for CSQ1 were highest in the EDL muscle, the relative CSQ1 protein levels in the soleus muscle were two times lower and the transcript levels more than 5 times lower compared to the EDL. In the left heart ventricle, protein isoform and CSQ1 transcript were also present, although at protein level, CSQ1 was hardly detectable. We suggest that the observed changes in CSQ1 levels in TH and HY compared to EU rats can be related to fiber type changes caused by alteration of the thyroid status rather than to the direct effect of thyroid hormones on CSQ1 gene expression
Permanent Link: http://hdl.handle.net/11104/0217454