Raman spectroscopy for bacterial identification and characterization

0388104 - ÚPT 2013 RIV US eng C - Conference Paper (international conference)
Bernatová, Silvie - Samek, Ota - Pilát, Zdeněk - Šerý, Mojmír - Ježek, Jan - Krzyžánek, Vladislav - Zemánek, Pavel - Růžička, F.
Raman spectroscopy for bacterial identification and characterization.
CPS 2012. 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE Vol.8697). Bellingham: SPIE, 2012, 86970Y: 1-6. ISBN 978-0-8194-9481-8.
[CPS 2012. Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics /18./. Ostravice (CZ), 03.09.2012-07.09.2012]
R&D Projects: GA MŠMT ED0017/01/01; GA ČR GAP205/11/1687; GA MŠMT EE2.4.31.0016
Institutional support: RVO:68081731
Keywords : Raman spectroscopy * Raman tweezers * bacteria * yeast biofilm * PCA
Subject RIV: BH - Optics, Masers, Lasers

The main goal of our investigation is to use Raman tweezers technique so that the responce of Raman scattering on microorganisms suspended in liquid media (bacteria, algae and yeast cells in microfluidic chips) can be used to identify different species. The investigations presented here include identification of different bacteria strains (biofilm-positive and biofilm-negative) and yeast cells by using principal component analysis (PCA). The main driving force behind our investigation was a common problem in the clinical microbiology laboratory - how to distinguish between contaminant and invasive isolates. Invasive bacterial/yeast isolates can be assumed to form a biofilm, while isolates which do not form a biofilm can be treated as contaminant. Thus, the latter do not represent an important virulence factor.
Permanent Link: http://hdl.handle.net/11104/0217086