The transmembrane region is responsible for targeting of adaptor protein LAX into "heavy rafts''

0387633 - ÚMG 2013 RIV US eng J - Journal Article
Hrdinka, Matouš - Otáhal, Pavel - Hořejší, Václav
The transmembrane region is responsible for targeting of adaptor protein LAX into "heavy rafts''.
PLoS ONE. Roč. 7, č. 5 (2012), e36330. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GEMEM/09/E011; GA MŠMT 1M0506
Institutional research plan: CEZ:AV0Z50520514
Keywords : LAX * transmembrane domain * DRM
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.730, year: 2012

The importance of membrane compartmentalization into specific membrane microdomains has been shown in many biological processes such as immunoreceptor signaling, membrane trafficking, pathogen infection, and tumor progression. Microdomains like lipid rafts, caveolae and tetraspanin enriched microdomains are relatively resistant to solubilization by some detergents. Large detergent-resistant membrane fragments (DRMs) resulting from such membrane solubilization can be conveniently isolated by density gradient ultracentrifugation or gel filtration. Recently, we described a novel type of raft-like membrane microdomains producing, upon detergent Brij98 solubilization, “heavy DRMs” and containing a number of functionally relevant proteins. Transmembrane adaptor protein LAX is a typical “heavy raft” protein. The present study was designed to identify the molecular determinants targeting LAX-derived constructs to heavy rafts. We identified the transmembrane domain of LAX as a first motif targeting transmembrane protein constructs to detergent-resistant heavy rafts, a novel type of membrane microdomains containing a number of physiologically important proteins.
Permanent Link: http://hdl.handle.net/11104/0216733