Surface plasmon resonance biosensor based on engineered proteins for direct detection of interferon-gamma in diluted blood plasma

0387101 - ÚFE 2013 RIV CH eng J - Journal Article
Šípová, Hana - Ševců, Veronika - Kuchař, Milan - Ahmad, Jawid Nazir - Mikulecký, Pavel - Osičková, Adriana - Malý, Petr - Homola, Jiří
Surface plasmon resonance biosensor based on engineered proteins for direct detection of interferon-gamma in diluted blood plasma.
Sensors and Actuators B - Chemical. Roč. 174, č. 11 (2012), s. 306-311. E-ISSN 0925-4005
R&D Projects: GA AV ČR KAN200670701
Institutional support: RVO:67985882 ; RVO:61388971 ; RVO:86652036
Keywords : Interferon gamma * Surface plasmon resonance * Biosensor
Subject RIV: JB - Sensors, Measurment, Regulation
Impact factor: 3.535, year: 2012

We present a novel surface plasmon resonance (SPR) biosensor for direct detection of human interferon gamma (hIFN). The biorecognition elements for hIFNare engineered proteins derived from albumin binding domain (ABD). Two methods for the immobilization of ABD are compared: covalent coupling and immobilization via streptavidin–biotin interaction. The developed biosensor provides rapid and sensitive platform for the detection of hIFN at subnanomolar levels both in buffer and diluted human plasma.
Permanent Link: http://hdl.handle.net/11104/0219884