Separation of clinically significant microorganisms from CSF by CIEF with UV detection and MALDI-TOF MS

0386072 - ÚIACH 2013 RIV CZ eng C - Conference Paper (international conference)
Horká, Marie - Šalplachta, Jiří - Kubesová, Anna - Růžička, F. - Šlais, Karel
Separation of clinically significant microorganisms from CSF by CIEF with UV detection and MALDI-TOF MS.
CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012 - (Foret, F.; Křenková, J.; Guttman, A.; Klepárník, K.; Boček, P.), s. 359-362. ISBN 978-80-904959-1-3.
[CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./. Brno (CZ), 01.11.2012-02.11.2012]
R&D Projects: GA MV VG20102015023
Institutional support: RVO:68081715
Keywords : capillary isoelectric focusing * labeled microorganisms * pre-concentration
Subject RIV: CB - Analytical Chemistry, Separation

The identification of most of the clinically significant species by the conventional laboratory methods is still time-consuming and often insufficient for ensuring early targeted therapy. This is especially important in the case of the nosocomial infections where the number of microorganisms in the cerebrospinal fluid is often ranging from units up to hundreds of cells per milliliter during bacterial infections of the central nervous system. The electrophoretic techniques with UV-detection reach the detection limit of intact cells approximately from 107 cells mL-1.
Permanent Link: http://hdl.handle.net/11104/0215758