Differences in fingerprints of biofilm-positive and biofilm-negative Candida strains exploitable for clinical practice

0386008 - ÚIACH 2013 RIV CZ eng C - Conference Paper (international conference)
Vykydalová, Marie - Horká, Marie - Šalplachta, Jiří - Růžička, E. - Kahle, Vladislav
Differences in fingerprints of biofilm-positive and biofilm-negative Candida strains exploitable for clinical practice.
CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012 - (Foret, F.; Křenková, J.; Guttman, A.; Klepárník, K.; Boček, P.), s. 117-120. ISBN 978-80-904959-1-3.
[CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./. Brno (CZ), 01.11.2012-02.11.2012]
R&D Projects: GA MV VG20112015021; GA MŠMT(CZ) EE2.3.20.0182
Institutional support: RVO:68081715
Keywords : Candida * biofilm * fingerprint
Subject RIV: CB - Analytical Chemistry, Separation

In this study, we have presented differences in fingerprints of Candida parapsilosis and Candida metapsilosis as well as their biofilm-positive and biofilm-negative forms. Fingerprints of yeasts were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis, capillary isoelectric focusing, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Samples for analyses were prepared by treatment of yeasts by boiling water or ethanol. Fingerprints of tested yeasts cells have differed in all cases. This knowledge can be used for obtaining rapid and simple method which distinguishes above mentioned yeasts in standard biochemical laboratories.
Permanent Link: http://hdl.handle.net/11104/0215227