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Capillary electrophoresis in classical and carrier ampholytes-based background electrolytes applied to separation and characterization of gonadotropin-releasing hormones
- 1.0385773 - ÚOCHB 2013 RIV NL eng J - Journal Article
Šolínová, Veronika - Poitevin, M. - Koval, Dušan - Busnel, J. M. - Peltre, G. - Kašička, Václav
Capillary electrophoresis in classical and carrier ampholytes-based background electrolytes applied to separation and characterization of gonadotropin-releasing hormones.
Journal of Chromatography A. Roč. 1267, SI (2012), s. 231-238. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR(CZ) GA203/08/1428
Grant - others:GA ČR(CZ) GAP206/12/0453
Program: GA
Institutional support: RVO:61388963
Keywords : CABCE * isoelectric background electrolytes * peptide hormones
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.612, year: 2012
Capillary zone electrophoresis (CZE) in classical buffer-based background electrolytes (BGEs) and carrier ampholytes-based capillary electrophoresis (CABCE) using narrow pH cuts of carrier ampholytes as constituents of quasi-isoelectric BGEs have been applied to separation and characterization of synthetic human and salmon gonadotropin-releasing hormones (GnRH) and their derivatives and fragments. The selectivity, separation efficiency, resolution and speed of CZE and CABCE analyses have been compared within a wide pH range of the BGEs (3.50 – 9.75) using two mixtures of structurally related GnRH peptides as model analytes.
Permanent Link: http://hdl.handle.net/11104/0215177
Number of the records: 1