Application of monolithic affinity HPLC column for rapid determination of malt glycoproteins

0384159 - ÚIACH 2013 RIV US eng J - Journal Article
Benkovská, Dagmar - Flodrová, Dana - Bobálová, Janette
Application of monolithic affinity HPLC column for rapid determination of malt glycoproteins.
Journal of Liquid Chromatography & Related Technologies. Roč. 36, č. 5 (2013), s. 561-572. ISSN 1082-6076. E-ISSN 1520-572X
R&D Projects: GA MŠMT 1M0570
Institutional research plan: CEZ:AV0Z4031919
Keywords : concanavalin A * glycosylation * barley
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 0.638, year: 2013

The aim of this study was to optimize separation and enrichment of barley malt glycoproteins on a monolithic ConA affinity HPLC column. ConA-bound proteins were separated on SDS-PAGE and identified using MALDI-TOF/TOF MS after chymotryptic digestion. Our proteomic analysis allowed successful determination of several putative malt glycoproteins.
Permanent Link: http://hdl.handle.net/11104/0215482