Review on recent advances in the analysis of isolated organelles

0383882 - ÚIACH 2013 RIV NL eng J - Journal Article
Satori, Ch. P. - Košťál, Vratislav - Arriaga, E. A.
Review on recent advances in the analysis of isolated organelles.
Analytica Chimica Acta. Roč. 753, NOV 13 (2012), s. 8-18. ISSN 0003-2670. E-ISSN 1873-4324
R&D Projects: GA ČR(CZ) GBP206/12/G014
Institutional research plan: CEZ:AV0Z40310501
Keywords : organelle isolation * fluorescence * electrophoresis
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.387, year: 2012

The analysis of isolated organelles is one of the pillars of modern bioanalytical chemistry. This review describes recent developments on the isolation and characterization of isolated organelles both from living organisms and cell cultures. Salient reports on methods to release organelles focused on reproducibility and yield, membrane isolation, and integrated devices for organelle release. New developments on organelle fractionation after their isolation were on the topics of centrifugation, immunocapture, free flow electrophoresis, flow field-flow fractionation, fluorescence activated organelle sorting, laser capture microdissection, and dielectrophoresis. New concepts on characterization of isolated organelles included atomic force microscopy, optical tweezers combined with Raman spectroscopy, organelle sensors, flow cytometry, capillary electrophoresis, and microfluidic devices.
Permanent Link: http://hdl.handle.net/11104/0215820