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Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA
- 1.0383454 - BFÚ 2013 RIV NL eng J - Journal Article
Bartošík, Martin - Fojta, Miroslav - Paleček, Emil
Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA.
Electrochimica acta. Roč. 78, SEP (2012), s. 75-81. ISSN 0013-4686. E-ISSN 1873-3859
R&D Projects: GA ČR(CZ) GAP301/11/2055; GA MŠMT(CZ) ME09038
Institutional research plan: CEZ:AV0Z50040702
Keywords : DNA methylation * 5-methylcytosine analysis * sodium bisulfite
Subject RIV: BO - Biophysics
Impact factor: 3.777, year: 2012
Using DNA bisulfite treatment, reducible cytosines were transformed into nonreducible uracil residues, strongly decreasing square wave voltammetric C reduction peaks of unmethylated DNA. On the other hand, 5-methylcytosines were not transformed and the reduction peaks of methylated DNA remained relatively unchanged. By combining DNA bisulfite treatment with square wave voltammetry, DNA methylation can be determined quantitatively at nanomolar and subnanomolar ODN concentrations.
Permanent Link: http://hdl.handle.net/11104/0213388
Number of the records: 1